Basic Local Alignment and Search Tool
Tutorial and training materials by OpenHelix
|Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.|
- how to do basic nucleotide and protein BLAST searches
- how to interpret and save your BLAST results
- how to manipulate your data for easy analysis and visualization
- to customize and save your searches and results with My NCBI
- to use some of the specialized BLAST tools for multiple alignments and SNP analysis
Recent BioMed Central research articles citing this resource
Bertrand Claudia et al., Mitochondrial and nuclear phylogenetic analysis with Sanger and next-generation sequencing shows that, in Área de Conservación Guanacaste, northwestern Costa Rica, the skipper butterfly named Urbanus belli (family Hesperiidae) comprises three morphologically cryptic species Phylogenetics and phylogeography. BMC Evolutionary Biology (2014) doi:10.1186/1471-2148-14-153
Jahn T Martin et al., A novel cost effective and high-throughput isolation and identification method for marine microalgae. Plant Methods (2014) doi:10.1186/1746-4811-10-26
Friedman-Einat Miriam et al., Quack leptin Non-human and non-rodent vertebrate genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-551
de Barros Lopes Lívia et al., Rickettsia bellii Rickettsia amblyommii , and Laguna Negra hantavirus in an Indian reserve in the Brazilian Amazon. Parasites Vectors (2014) doi:10.1186/1756-3305-7-191
Ohyama Hiromasa et al., The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8 Prokaryote microbial genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-386
More about the resource:
BLAST is a key resource developed and supported by the National Center for Biotechnology Information (NCBI). Sequence submissions from around the world help to populate NCBI's Genbank database, to which novel sequences can be compared using BLAST. BLAST results are linked to the vast NCBI Entrez network of databases allowing for a rich resource for discovery.
The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
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