Basic Local Alignment and Search Tool
Tutorial and training materials by OpenHelix
|Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.|
- how to do basic nucleotide and protein BLAST searches
- how to interpret and save your BLAST results
- how to manipulate your data for easy analysis and visualization
- to customize and save your searches and results with My NCBI
- to use some of the specialized BLAST tools for multiple alignments and SNP analysis
Recent BioMed Central research articles citing this resource
Cao Lihuan et al., Phylogenetic analysis of CDK and cyclin proteins in premetazoan lineages Genome evolution and evolutionary systems biology. BMC Evolutionary Biology (2014) doi:10.1186/1471-2148-14-10
Wang Linhai et al., Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis. Genome Biology (2014) doi:10.1186/gb-2014-15-2-r39
Pervez Sidra et al., Saccharification and liquefaction of cassava starch: an alternative source for the production of bioethanol using amylolytic enzymes by double fermentation process Protein and enzyme technology. BMC Biotechnology (2014) doi:10.1186/1472-6750-14-49
Liu Shikai et al., Development of the catfish 250K SNP array for genome-wide association studies. BMC Research Notes (2014) doi:10.1186/1756-0500-7-135
Ha Hongseok et al., A comprehensive analysis of piRNAs from adult human testis and their relationship with genes and mobile elements Human and rodent genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-545
More about the resource:
BLAST is a key resource developed and supported by the National Center for Biotechnology Information (NCBI). Sequence submissions from around the world help to populate NCBI's Genbank database, to which novel sequences can be compared using BLAST. BLAST results are linked to the vast NCBI Entrez network of databases allowing for a rich resource for discovery.
The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
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