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Basic Local Alignment and Search Tool

Tutorial and training materials by OpenHelix

Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.
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You'll learn:

  • how to do basic nucleotide and protein BLAST searches
  • how to interpret and save your BLAST results
  • how to manipulate your data for easy analysis and visualization
  • to customize and save your searches and results with My NCBI
  • to use some of the specialized BLAST tools for multiple alignments and SNP analysis


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Recent BioMed Central research articles citing this resource

Murukarthick Jayakodi et al., BrassicaTED - a public database for utilization of miniature transposable elements in Brassica species Bioinformatics. BMC Research Notes (2014) doi:10.1186/1756-0500-7-379

Ha Hongseok et al., A comprehensive analysis of piRNAs from adult human testis and their relationship with genes and mobile elements Human and rodent genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-545

Vester Kjølhede Jan et al., Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing. Microbial Cell Factories (2014) doi:10.1186/1475-2859-13-72

Ma Jun et al., mtDNA haplogroup and single nucleotide polymorphisms structure human microbiome communities Eukaryote microbial genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-257

Caron Yannick et al., New insight in lymnaeid snails (Mollusca, Gastropoda) as intermediate hosts of Fasciola hepatica (Trematoda, Digenea) in Belgium and Luxembourg. Parasites Vectors (2014) doi:10.1186/1756-3305-7-66

More about the resource:

BLAST is a key resource developed and supported by the National Center for Biotechnology Information (NCBI). Sequence submissions from around the world help to populate NCBI's Genbank database, to which novel sequences can be compared using BLAST. BLAST results are linked to the vast NCBI Entrez network of databases allowing for a rich resource for discovery.


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The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
Copyright 2009, OpenHelix, LLC.

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