Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.

You will learn:

  • how to do basic nucleotide and protein BLAST searches
  • how to interpret and save your BLAST results
  • how to manipulate your data for easy analysis and visualization
  • to customize and save your searches and results with My NCBI
  • to use some of the specialized BLAST tools for multiple alignments and SNP analysis
TUTORIAL RELATED CONTENT

TUTORIALS

This tutorial is a part of the tutorial group Alignment and comparison resources. You might find the other tutorials in the group interesting:

ClustalW2: Performs multiple sequence alignments

FASTA: FASTA sequence algorithm

Entrez Gene: NCBI's Entrez tool for gene-centric information

Primer3: Pick primers from a DNA sequence.

DCODE: DCODE.org Comparative Genomics Developments, a collection of powerful comparative genomics tools

VISTA: Tools for Comparative Genomics

RefSeq: Provides molecular sequence records to help locate gene and protein data.

CATEGORIES

Algorithms and Analysis : This category contains various tools that may help perform analysis of different genomics data types. This may include sequence alignment, large-scale or complex queries, motif finding, or comparative assessments.

NCBI : This category includes resources maintained at the National Center for Biotechnology Information (NCBI).

BLOG POSTS

Bioinformatics tools extracted from a typical mammalian genome project [supplement]: This is Table 1 that accompanies the full blog post: Bioinformatics tools extracted from a typical mammalian genome project. See the main post for the details and explanation. The table is too long to ...

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Video Tip of the Week: Sharing #H7N9 data at GISAID.org with EpiFlu™: This week’s video Tip of the Week offers you a quick tour of GISAID’s resources and their EpiFlu™ database. This is the database you might be hearing about in the news—the one to which researchers sub...

Friday SNPpets: Welcome to our Friday feature link collection: SNPpets. During the week we come across a lot of links and reads that we think are interesting, but don't make it to a blog post. Here they are for your e...

New Online Tutorials for Sequence Similarity Search Tools BLAST and FASTA: OpenHelix today announced the availability of new tutorial suites on two highly used sequence similarity search resources: BLAST and FASTA. BLAST, from the National Center for Biotechnology Information...

BIOMED CENTRAL

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