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Basic Local Alignment and Search Tool

Tutorial and training materials by OpenHelix

Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.
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You'll learn:

  • how to do basic nucleotide and protein BLAST searches
  • how to interpret and save your BLAST results
  • how to manipulate your data for easy analysis and visualization
  • to customize and save your searches and results with My NCBI
  • to use some of the specialized BLAST tools for multiple alignments and SNP analysis


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Recent BioMed Central research articles citing this resource

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Reis Silva Alexandre Libanio et al., Oxygen-limited cellobiose fermentation and the characterization of the cellobiase of an industrial Dekkera/Brettanomyces bruxellensis strain Biomedical and Life Sciences. SpringerPlus (2014) doi:10.1186/2193-1801-3-38

Wang Wei et al., A bovine viral diarrhea virus type 1a strain in China: isolation, identification, and experimental infection in calves Veterinary DNA viruses. Virology Journal (2014) doi:10.1186/1743-422X-11-8

Cao Xuan Hieu et al., Intergenomic single nucleotide polymorphisms as a tool for bacterial artificial chromosome contig building of homoeologous Brassica napus regions Plant genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-560

Martínez-López A Luis et al., Dynamics of the chili pepper transcriptome during fruit development Plant genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-143

More about the resource:

BLAST is a key resource developed and supported by the National Center for Biotechnology Information (NCBI). Sequence submissions from around the world help to populate NCBI's Genbank database, to which novel sequences can be compared using BLAST. BLAST results are linked to the vast NCBI Entrez network of databases allowing for a rich resource for discovery.


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The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
Copyright 2009, OpenHelix, LLC.

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