Basic Local Alignment and Search Tool
Tutorial and training materials by OpenHelix
|Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.|
- how to do basic nucleotide and protein BLAST searches
- how to interpret and save your BLAST results
- how to manipulate your data for easy analysis and visualization
- to customize and save your searches and results with My NCBI
- to use some of the specialized BLAST tools for multiple alignments and SNP analysis
Recent BioMed Central research articles citing this resource
Leggett M Richard et al., Reference-free SNP detection: dealing with the data deluge SNP-SIG 2013: Identification and annotation of genetic variants in the context of structure, function, and disease SNP-SIG 2013: Identification and annotation of genetic variants in the context of structure, function, and disease. BMC Genomics (2014) doi:10.1186/1471-2164-15-S4-S10
Wu Zhihua et al., Molecular evolution and functional characterisation of an ancient phenylalanine ammonia-lyase gene (NnPAL1) from Nelumbo nucifera: novel insight into the evolution of the PAL family in angiosperms Genome evolution and evolutionary systems biology. BMC Evolutionary Biology (2014) doi:10.1186/1471-2148-14-100
Dupeyron Mathilde et al., Horizontal transfer of transposons between and within crustaceans and insects. Mobile DNA (2014) doi:10.1186/1759-8753-5-4
Pereira LA Andre et al., Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response Plant genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-157
Mathew S Lisa et al., A first genetic map of date palm ( Phoenix dactylifera ) reveals long-range genome structure conservation in the palms Plant genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-285
More about the resource:
BLAST is a key resource developed and supported by the National Center for Biotechnology Information (NCBI). Sequence submissions from around the world help to populate NCBI's Genbank database, to which novel sequences can be compared using BLAST. BLAST results are linked to the vast NCBI Entrez network of databases allowing for a rich resource for discovery.
The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
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