Learn to use BLAST, (Basic Local Alignment and Search Tool), the sequence similarity searching tool from the National Center for Biotechnology Information (NCBI) at NIH. You can use BLAST to find sequences similar to your nucleotide or protein sequences. There are also a variety of specialized BLAST programs available, including Primer-BLAST, VecScreen, SNP BLAST options, multiple alignment tools, and more. You can easily compare 2 sequences to each other. These can provide invaluable information in your quest to infer functional and taxonomic relationships for known or unknown sequences, or to further design experiments.
You will learn:
This tutorial is a part of the tutorial group Alignment and comparison resources. You might find the other tutorials in the group interesting:
ClustalW2: Performs multiple sequence alignments
FASTA: FASTA sequence algorithm
Entrez Gene: NCBI's Entrez tool for gene-centric information
Primer3: Pick primers from a DNA sequence.
DCODE: DCODE.org Comparative Genomics Developments, a collection of powerful comparative genomics tools
VISTA: Tools for Comparative Genomics
RefSeq: Provides molecular sequence records to help locate gene and protein data.
Algorithms and Analysis : This category contains various tools that may help perform analysis of different genomics data types. This may include sequence alignment, large-scale or complex queries, motif finding, or comparative assessments.
NCBI : This category includes resources maintained at the National Center for Biotechnology Information (NCBI).
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Recent BioMed Central research articles citing this resource
Mosquera-Rendón Jeanneth et al., Pangenome-wide and molecular evolution analyses of the Pseudomonas aeruginosa species Prokaryote microbial genomics. BMC Genomics (2016) doi:10.1186/s12864-016-2364-4
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Tatarinova V. Tatiana et al., The mysterious orphans of Mycoplasmataceae. Biology Direct (2016) doi:10.1186/s13062-015-0104-3