Pick primers from a DNA sequence.
Tutorial and training materials by OpenHelix
|Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.|
- simple, basic primer selection
- to access more detailed primer design features, and check for mis-priming in your template
- to find the Primer3 source code, which can be used by programmers for many applications
Recent BioMed Central research articles citing this resource
Sherry Leighann et al., Biofilms formed by Candida albicans bloodstream isolates display phenotypic and transcriptional heterogeneity that are associated with resistance and pathogenicity Clinical microbiology and vaccines. BMC Microbiology (2014) doi:10.1186/1471-2180-14-182
Zhang Shuo et al., Development of highly polymorphic simple sequence repeat markers using genome-wide microsatellite variant analysis in Foxtail millet [ Setaria italica (L.) P. Beauv.] Plant genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-78
Pirooznia Mehdi et al., Validation and assessment of variant calling pipelines for next-generation sequencing. Human Genomics (2014) doi:10.1186/1479-7364-8-14
Mark J Peter et al., Rescue of glucocorticoid-programmed adipocyte inflammation by omega-3 fatty acid supplementation in the rat. Reproductive Biology and Endocrinology (2014) doi:10.1186/1477-7827-12-39
Kirov Ilya et al., An easy “SteamDrop” method for high quality plant chromosome preparation. Molecular Cytogenetics (2014) doi:10.1186/1755-8166-7-21
More about the resource:
The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.
The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
Copyright 2009, OpenHelix, LLC.