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Pick primers from a DNA sequence.

Tutorial and training materials by OpenHelix

Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.
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You'll learn:

  • simple, basic primer selection
  • to access more detailed primer design features, and check for mis-priming in your template
  • to find the Primer3 source code, which can be used by programmers for many applications


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Recent BioMed Central research articles citing this resource

Leung P Kai et al., Dermal wound transcriptomic responses to Infection with Pseudomonas aeruginosa versus Klebsiella pneumoniae in a rabbit ear wound model. BMC Clinical Pathology (2014) doi:10.1186/1472-6890-14-20

Porter Emily et al., Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis. Veterinary Research (2014) doi:10.1186/1297-9716-45-49

Stapp D Andrea et al., Evaluation of steroidogenic capacity after follicle stimulating hormone stimulation in bovine granulosa cells of Revalor 200 implanted heifers. Journal of Animal Science and Biotechnology (2014) doi:10.1186/2049-1891-5-2

Revilla Manuel et al., New insight into the SSC8 genetic determination of fatty acid composition in pigs. Genetics Selection Evolution (2014) doi:10.1186/1297-9686-46-28

Liu Bobin et al., WUSCHEL-related Homeobox genes in Populus tomentosa : diversified expression patterns and a functional similarity in adventitious root formation Plant genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-296

More about the resource:

The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.


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