Pick primers from a DNA sequence.
Tutorial and training materials by OpenHelix
|Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.|
- simple, basic primer selection
- to access more detailed primer design features, and check for mis-priming in your template
- to find the Primer3 source code, which can be used by programmers for many applications
Recent BioMed Central research articles citing this resource
Gutierrez-Gonzalez J Juan et al., Analysis and annotation of the hexaploid oat seed transcriptome Plant genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-471
Ribas Laia et al., A combined strategy involving Sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot ( Scophthalmus maximus Non-human and non-rodent vertebrate genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-180
Patade Vikas Yadav et al., Cold tolerance in Osmotin transgenic tomato ( Solanum lycopersicum L.) is associated with modulation in transcript abundance of stress responsive genes Biomedical and Life Sciences. SpringerPlus (2013) doi:10.1186/2193-1801-2-117
Ma Junwu et al., Fine mapping of fatness QTL on porcine chromosome X and analyses of three positional candidate genes Complex traits and quantitative genetics. BMC Genetics (2013) doi:10.1186/1471-2156-14-46
Wrzesinski H Stephen et al., Genetic profiles of plasmacytoid (BDCA-4 expressing) DC subtypes-clues to DC subtype function in vivo. Experimental Hematology Oncology (2013) doi:10.1186/2162-3619-2-8
More about the resource:
The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.
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