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Pick primers from a DNA sequence.

Tutorial and training materials by OpenHelix

Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.
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You'll learn:

  • simple, basic primer selection
  • to access more detailed primer design features, and check for mis-priming in your template
  • to find the Primer3 source code, which can be used by programmers for many applications


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Recent BioMed Central research articles citing this resource

Whelehan J Cormac et al., Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue Non-human and non-rodent vertebrate genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-128

Biselli Chiara et al., Improvement of marker-based predictability of Apparent Amylose Content in japonica rice through GBSSI allele mining Regular submissions. Rice (2014) doi:10.1186/1939-8433-7-1

Arnal Audrey et al., Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa). BMC Research Notes (2014) doi:10.1186/1756-0500-7-321

Nagarajha Selvan Dhevi Lakshmi et al., Proteogenomic analysis of pathogenic yeast Cryptococcus neoformans using high resolution mass spectrometry. Clinical Proteomics (2014) doi:10.1186/1559-0275-11-5

Serrat Xavier et al., EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations. Plant Methods (2014) doi:10.1186/1746-4811-10-5

More about the resource:

The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.


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