Pick primers from a DNA sequence.
Tutorial and training materials by OpenHelix
|Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.|
- simple, basic primer selection
- to access more detailed primer design features, and check for mis-priming in your template
- to find the Primer3 source code, which can be used by programmers for many applications
Recent BioMed Central research articles citing this resource
Mankotia Singh Dhananjay et al., Cloning and expression of N22 region of Torque Teno virus (TTV) genome and use of peptide in developing immunoassay for TTV antibodies Hepatitis viruses. Virology Journal (2014) doi:10.1186/1743-422X-11-96
Fanelli Roberto et al., Effects of a novel cyclic RGD peptidomimetic on cell proliferation, migration and angiogenic activity in human endothelial cells. Vascular Cell (2014) doi:10.1186/2045-824X-6-11
Attard Agnès et al., Transcriptome dynamics of Arabidopsis thaliana root penetration by the oomycete pathogen Phytophthora parasitica Eukaryote microbial genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-538
Modak Rahul et al., Epigenetic response in mice mastitis: Role of histone H3 acetylation and microRNA(s) in the regulation of host inflammatory gene expression during Staphylococcus aureus infection. Clinical Epigenetics (2014) doi:10.1186/1868-7083-6-12
Candeias Rui et al., Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba. BMC Research Notes (2014) doi:10.1186/1756-0500-7-73
More about the resource:
The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.
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