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Pick primers from a DNA sequence.

Tutorial and training materials by OpenHelix

Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.
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You'll learn:

  • simple, basic primer selection
  • to access more detailed primer design features, and check for mis-priming in your template
  • to find the Primer3 source code, which can be used by programmers for many applications


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Recent BioMed Central research articles citing this resource

Cai Qing et al., Multi-locus phylogeny of lethal amanitas: Implications for species diversity and historical biogeography Phylogenetics and phylogeography. BMC Evolutionary Biology (2014) doi:10.1186/1471-2148-14-143

Karsani Anuar Saiful et al., Comparative proteomics analysis of oral cancer cell lines: identification of cancer associated proteins. Proteome Science (2014) doi:10.1186/1477-5956-12-3

Luz-Madrigal Agustin et al., Reprogramming of the chick retinal pigmented epithelium after retinal injury. BMC Biology (2014) doi:10.1186/1741-7007-12-28

Kolokoltsova A Olga et al., Reactive astrogliosis in response to hemorrhagic fever virus: microarray profile of Junin virus-infected human astrocytes Negative-strand RNA viruses. Virology Journal (2014) doi:10.1186/1743-422X-11-126

Ji Zhisong et al., Novel loss-of-function PRRT2 mutation causes paroxysmal kinesigenic dyskinesia in a Han Chinese family Neurogenetics. BMC Neurology (2014) doi:10.1186/1471-2377-14-146

More about the resource:

The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.


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