Pick primers from a DNA sequence.
Tutorial and training materials by OpenHelix
| Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices. | Advertisement:
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You'll learn:
- simple, basic primer selection
- to access more detailed primer design features, and check for mis-priming in your template
- to find the Primer3 source code, which can be used by programmers for many applications
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Recent BioMed Central research articles citing this resourceSarika et al., In silico mining of putative microsatellite markers from whole genome sequence of water buffalo ( Bubalus bubalis ) and development of first BuffSatDB Non-human and non-rodent vertebrate genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-43 Ding Liang-Hao et al., Distinct transcriptome profiles identified in normal human bronchial epithelial cells after exposure to γ-rays and different elemental particles of high Z and energy Human and rodent genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-372 Rahman Obaid Ur et al., Deletion mutation in BSCL2 gene underlies congenital generalized lipodystrophy in a Pakistani family. Diagnostic Pathology (2013) doi:10.1186/1746-1596-8-78 Ngwa Che Julius et al., Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito Eukaryote microbial genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-256 Kuenne Carsten et al., Reassessment of the Listeria monocytogenes pan-genome reveals dynamic integration hotspots and mobile genetic elements as major components of the accessory genome Comparative and evolutionary genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-47 |
More about the resource:
The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.
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