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Pick primers from a DNA sequence.

Tutorial and training materials by OpenHelix

Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.
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You'll learn:

  • simple, basic primer selection
  • to access more detailed primer design features, and check for mis-priming in your template
  • to find the Primer3 source code, which can be used by programmers for many applications


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Recent BioMed Central research articles citing this resource

Kersting Sebastian et al., Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria Journal (2014) doi:10.1186/1475-2875-13-99

Karsani Anuar Saiful et al., Comparative proteomics analysis of oral cancer cell lines: identification of cancer associated proteins. Proteome Science (2014) doi:10.1186/1477-5956-12-3

Roelofs MJ Hennie et al., Over-expression of COX-2 mRNA in colorectal cancer Colorectal cancer. BMC Gastroenterology (2014) doi:10.1186/1471-230X-14-1

Leonel Camila et al., Expression of glutathione, glutathione peroxidase and glutathione S-transferase pi in canine mammary tumors Oncology. BMC Veterinary Research (2014) doi:10.1186/1746-6148-10-49

Cosson Patrick et al., Development and characterization of 96 microsatellite markers suitable for QTL mapping and accession control in an Arabidopsis core collection. Plant Methods (2014) doi:10.1186/1746-4811-10-2

More about the resource:

The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.


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