Pick primers from a DNA sequence.
Tutorial and training materials by OpenHelix
|Learn to use Primer3, a handy tool for primer design. Oligonucleotide primers are widely used in molecular biology applications, and Primer3 offers many ways to identify appropriate oligos for your research. Simple, quick primer designs are possible--as well as options for more detailed primer characteristics which are available to fine tune your primer choices.|
- simple, basic primer selection
- to access more detailed primer design features, and check for mis-priming in your template
- to find the Primer3 source code, which can be used by programmers for many applications
Recent BioMed Central research articles citing this resource
Sato Kazuki et al., Activated and inactivated immune responses in Caenorhabditis elegans against Photorhabdus luminescens TT01 Biomedical and Life Sciences. SpringerPlus (2014) doi:10.1186/2193-1801-3-274
Behlouli Asma et al., EPS8 , encoding an actin-binding protein of cochlear hair cell stereocilia, is a new causal gene for autosomal recessive profound deafness. Orphanet Journal of Rare Diseases (2014) doi:10.1186/1750-1172-9-55
Jebbawi Fadi et al., A microRNA profile of human CD8 regulatory T cells and characterization of the effects of microRNAs on Treg cell-associated genes. Journal of Translational Medicine (2014) doi:10.1186/s12967-014-0218-x
Bassett Leavel Carole et al., Genes responding to water deficit in apple ( Malus domestica Borkh.) roots Plant-abiotic interactions. BMC Plant Biology (2014) doi:10.1186/1471-2229-14-182
Wen Hongxiu et al., Family-specific, novel, deleterious germline variants provide a rich resource to identify genetic predispositions for BRCAx familial breast cancer Genetics, genomics and epigenetics. BMC Cancer (2014) doi:10.1186/1471-2407-14-470
More about the resource:
The Primer3 tool, which is used for the design of primers (oligos) for molecular biology research, was developed by Steve Rozen and Helen Skaletsky at MIT. This software helps researchers to generate PCR primers, sequencing primers, hybridization oligos--or for your short oligonucleotide fragment needs of almost any sort. This tool is freely available to the public, and can be found not only at the MIT Whitehead Institute website, but installed locally in many other institutions and companies as well. All of these Primer3 sites include software developed by the Whitehead Institute for Biomedical Research.
The materials and slides offered can not be resold or used for profit purposes. Reproduction, distribution and/or use is strictly limited to instructional purposes only and can not be used for for monetary gain or wide distribution.
Copyright 2009, OpenHelix, LLC.