Performs multiple sequence alignments
Tutorial and training materials by OpenHelix
|Learn to use the ClustalW2 resource at the EBI Toolbox site for performing multiple sequence alignments, or MSA. MSA allow us to analyze the evolutionary relationship between species on a molecular sequence level. As well, multiple sequence alignments can help us to identify functionally important positions in a sequence family by simply looking at the conservation of positions or motifs within the sequence data.|
Download the example data file by clicking here.
- some background and theory for multiple sequence alignments
- how to conduct sequence alignments using
- to understand and adjust the results of ClustalW2 alignments
Recent BioMed Central research articles citing this resource
Dela-Moss I Lumbini et al., Identification of conserved RNA secondary structures at influenza B and C splice sites reveals similarities and differences between influenza A, B, and C. BMC Research Notes (2014) doi:10.1186/1756-0500-7-22
Jabbari Neda et al., Sequence variation among members of the miR-200 microRNA family is correlated with variation in the ability to induce hallmarks of mesenchymal-epithelial transition in ovarian cancer cells. Journal of Ovarian Research (2014) doi:10.1186/1757-2215-7-12
Chaw Crystal R et al., Intragenic homogenization and multiple copies of prey-wrapping silk genes in Argiope garden spiders Genome evolution and evolutionary systems biology. BMC Evolutionary Biology (2014) doi:10.1186/1471-2148-14-31
Mennigen A Jan et al., Metabolic consequences of microRNA-122 inhibition in rainbow trout, Oncorhynchus mykiss Non-human and non-rodent vertebrate genomics. BMC Genomics (2014) doi:10.1186/1471-2164-15-70
Mariotti T Evan et al., Canine pulmonary adenocarcinoma tyrosine kinase receptor expression and phosphorylation Oncology. BMC Veterinary Research (2014) doi:10.1186/1746-6148-10-19
More about the resource:
ClustalW2 web-based interface featured in this tutorial is provided by the European Bioinformatics Institute (EBI). The Clustal program was originally published by Thompson et al from Toby Gibson’s group in 1994, and was later updated by Larkin et al. ClustalW can be downloaded as source code or as binaries for various operating systems at www.clustal.org, provided by the Conway Institute at the University College of Dublin.
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