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OpenHelix

Performs multiple sequence alignments

Tutorial and training materials by OpenHelix

Learn to use the ClustalW2 resource at the EBI Toolbox site for performing multiple sequence alignments, or MSA. MSA allow us to analyze the evolutionary relationship between species on a molecular sequence level. As well, multiple sequence alignments can help us to identify functionally important positions in a sequence family by simply looking at the conservation of positions or motifs within the sequence data.
Download the example data file by clicking here.
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You'll learn:

  • some background and theory for multiple sequence alignments
  • how to conduct sequence alignments using
  • to understand and adjust the results of ClustalW2 alignments


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Recent BioMed Central research articles citing this resource

Sierocka Izabela et al., Female-specific gene expression in dioecious liverwort Pellia endiviifolia is developmentally regulated and connected to archegonia production Reproductive biology. BMC Plant Biology (2014) doi:10.1186/1471-2229-14-168

Micallef L Melinda et al., Comparative analysis of hapalindole, ambiguine and welwitindolinone gene clusters and reconstitution of indole-isonitrile biosynthesis from cyanobacteria. BMC Microbiology (2014) doi:10.1186/s12866-014-0213-7

Huh Un Sung et al., APUM5, encoding a Pumilio RNA binding protein, negatively regulates abiotic stress responsive gene expression Plant-abiotic interactions. BMC Plant Biology (2014) doi:10.1186/1471-2229-14-75

Muñoz-Clares A Rosario et al., Exploring the evolutionary route of the acquisition of betaine aldehyde dehydrogenase activity by plant ALDH10 enzymes: implications for the synthesis of the osmoprotectant glycine betaine Genomics and evolution. BMC Plant Biology (2014) doi:10.1186/1471-2229-14-149

Okamoto Kenji et al., Bioconversion of xylose, hexoses and biomass to ethanol by a new isolate of the white rot basidiomycete Trametes versicolor Biomedical and Life Sciences. SpringerPlus (2014) doi:10.1186/2193-1801-3-121

More about the resource:

ClustalW2 web-based interface featured in this tutorial is provided by the European Bioinformatics Institute (EBI). The Clustal program was originally published by Thompson et al from Toby Gibsons group in 1994, and was later updated by Larkin et al. ClustalW can be downloaded as source code or as binaries for various operating systems at www.clustal.org, provided by the Conway Institute at the University College of Dublin.


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