Performs multiple sequence alignments
Tutorial and training materials by OpenHelix
|Learn to use the ClustalW2 resource at the EBI Toolbox site for performing multiple sequence alignments, or MSA. MSA allow us to analyze the evolutionary relationship between species on a molecular sequence level. As well, multiple sequence alignments can help us to identify functionally important positions in a sequence family by simply looking at the conservation of positions or motifs within the sequence data.|
Download the example data file by clicking here.
- some background and theory for multiple sequence alignments
- how to conduct sequence alignments using
- to understand and adjust the results of ClustalW2 alignments
Recent BioMed Central research articles citing this resource
Pan Guoqing et al., Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation Eukaryote microbial genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-186
Tang Le et al., Defining natural species of bacteria: clear-cut genomic boundaries revealed by a turning point in nucleotide sequence divergence Comparative and evolutionary genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-489
Zheng Wei Hao et al., Evolutionary relationships of ATP-Binding Cassette (ABC) uptake porters Microbial biochemistry, physiology and metabolism. BMC Microbiology (2013) doi:10.1186/1471-2180-13-98
Catalano Andrew et al., Rad53 homologue forkhead-associated kinase A (FhkA) and Ca 2+ -binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium. Cell Division (2013) doi:10.1186/1747-1028-8-4
Navabi Zahra-Katy et al., Conserved microstructure of the Brassica B Genome of Brassica nigra in relation to homologous regions of Arabidopsis thaliana B. rapa and B. oleracea Plant genomics. BMC Genomics (2013) doi:10.1186/1471-2164-14-250
More about the resource:
ClustalW2 web-based interface featured in this tutorial is provided by the European Bioinformatics Institute (EBI). The Clustal program was originally published by Thompson et al from Toby Gibson’s group in 1994, and was later updated by Larkin et al. ClustalW can be downloaded as source code or as binaries for various operating systems at www.clustal.org, provided by the Conway Institute at the University College of Dublin.
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