The RefSeq project, developed and maintained by the National Center for Biotechnology Information (NCBI), provides collected, curated, and consistent non-redundant reference molecular sequence records that help researchers locate important gene and protein data. Processes established by the RefSeq team generate reference sequence sets for many species. RefSeq records link to primary source material, appropriate literature, and additional information from other biomedical resources. Expanded project directions offer more data types. RefSeq, and similar projects with ties to RefSeq, continue to provide quality, reviewed, reference sequences for a variety of projects. RefSeq data can be found in many bioinformatics tools.

You will learn:

  • The history and framework for RefSeq
  • How to find and examine RefSeq data at NCBI
  • The composition of a typical RefSeq record
  • About other project directions and related projects


This tutorial is a part of the tutorial group Alignment and comparison resources. You might find the other tutorials in the group interesting:

ClustalW2: Performs multiple sequence alignments

BLAST: Basic Local Alignment and Search Tool

FASTA: FASTA sequence algorithm

Entrez Gene: NCBI's Entrez tool for gene-centric information

Primer3: Pick primers from a DNA sequence.

DCODE: Comparative Genomics Developments, a collection of powerful comparative genomics tools

VISTA: Tools for Comparative Genomics


Nucleotides : Tools that are primarily used in the storage, retrieval, or exploration of nucleotide-based data. Some tools may also involve protein sequence information.

NCBI : This category includes resources maintained at the National Center for Biotechnology Information (NCBI).


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